The bank is thought to have broken sanctions against Iran, according to reports by the Financial Times and Reuters, although this has not been confirmed by Unicredit.
The probe centres on a German subsidiary, HypoVereinsbank, which the major Italian bank bought in 2005.
The news follows similar revelations about two UK banks.
Unicredit originally admitted in January as part of a regulatory filing that it was working with US authorities over a sanctions breach, but without naming the country involved.
“A member of the Unicredit group is currently responding to a third party witness subpoena from the New York County District Attorney’s Office in connection with an ongoing investigation regarding certain persons and/or entities believed to have engaged in sanctionable activities,” the January filing said.
According to Unicredit’s latest statement, which does not name Iran either, the investigation is also being conducted by the US Department of Justice.
Last month the US Senate released a report detailing how HSBC helped launder money for Iran, as well as for other US-sanctioned governments of Burma and North Korea and for Mexican drugs cartels.
Then, earlier this month, Standard Chartered Bank – which is headquartered in London, but mainly active in the Middle East, Africa and Asia – agreed to pay New York regulators $340m (£217m) to settle claims that it had concealed $250bn in transactions with Iran.
Who is under scrutiny?
Banks around the world are facing allegations of inter-bank rate rigging, lax anti-money laundering controls and contravening economic sanctions. But which banks are in the frame, for what – and by which regulators?
Meanwhile, Royal Bank of Scotland is also understood to be facing investigations into whether it has broken sanctions against Iran.
The bank would not comment, but confirmed that it had voluntarily approached US and UK officials with information after an internal inquiry uncovered possible infringements.
Germany’s Commerzbank also warned last week that it may have to make a hefty payment to settle a US investigation into its own violations of sanctions on Iran and other countries.
Press reports earlier this month suggested that another German bank, Deutsche Bank, is also being investigated by the US Treasury’s Office of Foreign Assets Control, the Federal Reserve, the US Justice Department and Manhattan’s district attorney’s office for alleged infringements of US-Iran economic sanctions.
Deutsche Bank refused to comment on the reports.
Iran has been subject to US economic sanctions since 1979. The current system operates under the US Treasury Department’s Office of Foreign Assets Control.
The sanctions were toughened in 1997 by then-President Bill Clinton, who signed an order for sanctions that prohibited “virtually all trade and investment activities with Iran by US persons, wherever located”.
Under US criminal law, violations of the Iranian Transactions Regulations may result in a fine up to $1m and/or jail for up to 20 years.
As part of the sanctions regime, until 2008, banks in the US in some circumstances were allowed to undertake so-called U-turn transactions with Iranian financial institutions.
Those U-turn transactions move money for Iranian clients among non-Iranian foreign banks, such as those in the UK and the Middle East. They are cleared through the US, but neither start nor end in Iran.
To ascertain whether these transactions are permitted, US clearing banks use the wire-transfer messages they get from banks, using the SWIFT payments system.
If the banks do not have enough information to make the call, they are supposed to freeze the assets.
The allegations involving Standard Chartered and HSBC both centred on U-turn transactions.
Standard Chartered was accused of stripping the messages of data that showed the clients were Iranian, replacing it with false entries.
The UK-based bank said that not only did “99.9% of the transactions” relating to Iran comply with U-turn regulations, but that the total value of transactions that did not comply was under $14m – converse to the $250bn worth of Iran transactions US regulators said it had hidden.
In July, a US Senate Committee found that HSBC carried out 25,000 transactions totalling $19bn that were connected to Iran between 2001-07, which it suggested was evidence that the bank may have broken economic sanctions.